Transcription Initiation at Its Most Basic Level

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Transcription Initiation at Its Most Basic Level

The structure of the initiator binding protein from the primitive eukaryote Trichomonas vaginalis reveals how a single protein can orchestrate promoter recognition, RNA polymerase II recruitment, and start site selection, and provides an evolutionary link with transcription initiation in higher eukaryotes.

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A Chromatin Landmark and Transcription Initiation at Most Promoters in Human Cells

We describe the results of a genome-wide analysis of human cells that suggests that most protein-coding genes, including most genes thought to be transcriptionally inactive, experience transcription initiation. We found that nucleosomes with H3K4me3 and H3K9,14Ac modifications, together with RNA polymerase II, occupy the promoters of most protein-coding genes in human embryonic stem cells. Only...

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Transcription Initiation Patterns Indicate Divergent Strategies for Gene Regulation at the Chromatin Level

The application of deep sequencing to map 5' capped transcripts has confirmed the existence of at least two distinct promoter classes in metazoans: "focused" promoters with transcription start sites (TSSs) that occur in a narrowly defined genomic span and "dispersed" promoters with TSSs that are spread over a larger window. Previous studies have explored the presence of genomic features, such a...

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transcription initiation

Ve showed earlier [101 that the region of the bla promoter of Tn3 protected by the RHA-polymerase (REAP), has the normal size (about 60bp) at RJAP/promoter molar ratio r(2, but rises to about twice this extent as r increases. Ve confirm here that the species corresponding to normal and extended footprint distinguish by their electrophoretic mobilities. Furtheraore, inspection of the complexes b...

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ژورنال

عنوان ژورنال: Cell

سال: 2003

ISSN: 0092-8674

DOI: 10.1016/s0092-8674(03)00888-2